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SDS-PAGE of 53D1 purification. CE, crude extract, i.e., S. coelicolor/pIJ86::53D1 concentrated culture broth, loaded onto HiTrap-Chelating affinity column; 1, fractions collected from the initial washing of the column; 2, purified 53D1; Std, standard reference proteins. 53D1 protein band is indicated with an arrow.
