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. 2019 Jun 18;10:1358. doi: 10.3389/fmicb.2019.01358

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Copyright © 2019 Berini, Casartelli, Montali, Reguzzoni, Tettamanti and Marinelli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Residual chitinolytic activities of 53D1 (A) and Chi18H8 (B), incubated at 25°C in Tris–HCl pH 8.0 (control, •, solid line) or in the presence of undiluted (, dotted line), 10-fold diluted (, dotted line), or 100-fold diluted (, dotted line) midgut juice from B. mori larvae. Enzyme aliquots were collected at increasing time intervals and the residual activity was measured on 4-MU-(GlcNAc)₂ as substrate.

Inline graphic — Residual chitinolytic activities of 53D1 (A) and Chi18H8 (B), incubated at 25°C in Tris–HCl pH 8.0 (control, •, solid line) or in the presence of undiluted (, dotted line), 10-fold diluted (, dotted line), or 100-fold diluted (, dotted line) midgut juice from B. mori larvae. Enzyme aliquots were collected at increasing time intervals and the residual activity was measured on 4-MU-(GlcNAc)₂ as substrate.