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. 2019 Jun 18;10:1336. doi: 10.3389/fimmu.2019.01336

Figure 1.

Figure 1

CD4+ and CD8+ blood T cells of melanoma patients show TCR repertoire restrictions with clonal expansions. (A) Definitions of non-restricted/restricted Vβ-chain and distinct peak. (B) Representative CDR3 spectratypes determined for 27 different Vβ-gene families from CD4+ and CD8+ peripheral blood lymphocytes (PBL) of healthy controls (n = 8) and patients with advanced melanoma (n = 20). Vβ15 spectratyping is shown in 27 bp width and Vβ23 in 24 bp width. (C,D) Numbers of restricted Vβ-gene families or distinct peaks in spectratypes of CD4+ (C) and CD8+ (D) blood T cells are compared by Mann-Whitney U-test. Each dot represents one subject. (E,F) Sequencing of TCR β-chain rearrangements from select TCR Vβ-gene families identified clonal TCRs in CD4+ (E) and CD8+ (F) blood T cells of melanoma patients. Each slice represents a TCR Vβ-gene rearrangement. White slices represent sequences found only once. Identical TCR rearrangements are color-highlighted. CDR3 amino acid sequences (one letter code) and rearranged Jβ genes are given. (G,H) Sequencing of TCR β-chain rearrangements of CD4+ (G) and CD8+ (H) cells from blood and tumor-infiltrating lymphocytes (TILs) identified common clones.