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. 2019 Jun 18;10:1336. doi: 10.3389/fimmu.2019.01336

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Copyright © 2019 Arakawa, Vollmer, Tietze, Galinski, Heppt, Bürdek, Berking and Prinz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pretreatment TCR repertoire restrictions were not different in patient groups with different ages, sex, metastatic organ, or dacarbazine treatment. (A,C,D,E) Numbers of restricted Vβ-gene families or distinct peaks in spectratypes of CD4⁺ and CD8⁺ blood T cells are compared between patient groups with different age (A), sex (C), stage (D) or preimmunotherapy dacarbazine treatment (E) by Mann-Whitney U-test. Data in Figures 1C,D are re-analyzed. (B) TCR repertoires are compared between healthy controls and 8 youngest melanoma patients so that there are no statistical differences in age between groups.