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. 2019 Jun 18;10:1363. doi: 10.3389/fmicb.2019.01363

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Copyright © 2019 Jeong, Kim, Kim, Yun and Han.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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S. aureus with D-alanine-deficient LTA and WTA is susceptible to the growth inhibition by NaP. (A) WT, (B) ΔltaS, (C) ΔtagO, (D) Δlgt, (E) ΔdltA, (F) ΔltaS/pM101-ltaS, (G) ΔtagO/pStagO, (H) Δlgt/pSlgt or (I) ΔdltA/p0793 was inoculated and cultured in the presence or absence of 12.5 mM of NaA, NaP, or NaB. The optical density at 600 nm was measured hourly. (J–L) The MIC/MBC test for NaP was conducted using the microdilution method with (J) WT, (K) ΔdltA, or (L) ΔdltA/p0793. The MIC, the concentration of NaP which completely inhibited growth, is indicated with an arrow. Data shown are the mean values ± SD of triplicate samples and are representative of at least three similar independent experiments.