Figure 5.
NaP reduces bacterial load in murine skin infection when co-treated with a D-alanylation inhibitor. C57BL/6 mice were subcutaneously infected with 3 × 106 CFU MRSA USA300 together with 50 mM NaP (left), or with 50 mM NaP and 10 μg/ml AMSA (right) (n = 10). (A) Images of abscesses on day 3. Scale bar indicates 1 cm. On day 3, after euthanasia, (B) size, and (C) weight of abscesses were measured. (D) Bacterial load was measured by excising and homogenizing abscesses aseptically, and spotting homogenates on TSB agar plates. Homogenates were centrifuged and the supernatants were used to measure (E) IL-1β and (F) IL-6. Data are represented as mean values ± SEM, and statistical significance was measured with the student’s t-test. ∗P < 0.05.