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. 2019 Jun 18;10:765. doi: 10.3389/fphys.2019.00765

FIGURE 1.

FIGURE 1

miR-10 expression in developing testes and generation of germ cells miR-10a conditional overexpression transgenic mice. (A,B) RT-qPCR analyses of the expression levels of miR-10a (A) and miR-10b (B) in different ages of mouse testes. The miR-10a/b expression is relative to U6 and data are presented as mean ± SEM (n = 3). (C) Strategy for generating a miR-10a overexpressed construct in mice. In the targeting allele, a modified vector CMV-LoxP-GFP-STOP-LoxP containing a synthetic rTA-polyA-TetO-CMV element was insert into upstream of pri-miR-10a, and Cre-mediated recombination leads to the deletion of STOP cassette, which causes pri-miR-10a transcript into mature miR-10a. (D) Breeding scheme for germ cells specific miR-10a overexpression mouse model by Ddx4-Cre mediation and representative PCR based genotyping results. (E) RT-qPCR analysis showing the miR-10a expression level at P7, P10, P14, P21 and adult testes from miR-10a conditional overexpressed (Ddx4-cOE) and WT mice. Data are presented as mean ± SEM (n = 3), ∗∗P < 0.01.