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. 2019 Jun 18;10:765. doi: 10.3389/fphys.2019.00765

FIGURE 5.

FIGURE 5

Rad51 as a key target of miR-10a in vivo is essential for male fertility in both mouse and human. (A) Protein level of RAD51 in WT and Ddx4-cOE testes at P6, P10, and P14 by Western blot and protein quantification analyses, respectively. Protein quantification data are presented as mean ± SEM (n = 3). β-ACTIN as loading control. *P < 0.05, ∗∗P < 0.01. (B–D) Histology and hsa-miR-10a/b expression level analyses of testis biopsy tissues from six non-obstructive azoospermia (NOA) patients (NOA#1-6) and one OA patient (as control) by PAS-staining and RT-qPCR. Note that NOA patient #1, 2, and 5 are all showing Sertoli cell only (SCO) phenotype (B), NOA patient #3 and 6 showing post-meiotic arrested phenotype (D). Both hsa-miR-10a and hsa-miR-10b expression levels were showing up-regulated in patient #3 and 6 (C). The experiments were performed technically triplicates. *P < 0.05. Scale bar = 50 μm. NM means normal testis sections from fertile men. (E) RT-qPCR analysis of Rad51 mRNA levels in NOA patients #3, #6, OA patients, respectively. The experiments were performed technically triplicates. ∗∗P < 0.01. (F) A schematic model showing miR-10a regulation of meiotic process in spermatocytes by direct targeting Rad51.