FIGURE 2.

CADM1 is enriched at mast cell-sensory neurite contact sites. Representative immunofluorescent images of BMMCs co-cultured with DRG neurons for 24 h. In panel (A) individual and overlaid images are shown from a neurite with two adherent cells. β-Tubulin (red) is used as the neuronal marker, DAPI (blue) nuclear marker labels all cells in the culture, while FcεRI (yellow) specifically labels BMMCs. CADM1 (green) stained positive in the sensory neuron neurite and BMMC, but is absent from the attached non-neuronal, non-mast cell. (B,C) Representative individual and overlaid immunofluorescent images of CADM1 (green), β-Tubulin (red) and DAPI (blue) staining of different co-cultures. Increased CADM1 fluorescent intensity at contact sites (white arrows) between BMMCs and neurites (red).