Figure 3.

B. adolescentis reversed the imbalance of the intestinal microflora induced by CRS at the phylum level. The mice in the Con, CRS, and Bif+CRS groups were given 10 mL/kg distilled water, 10 mL/kg distilled water, and 0.25 × 10⁹ CFU/kg B. adolescentis, respectively, by gavage for 21 days. (A) There was no significant difference in the Shannon index at the phylum level induced by B. adolescentis. (B) The principal component analysis (PCA) results showed no significant difference in the microbial community composition. (C) Community barplot analysis showed the community composition and species abundance in the three groups. (D) The decrease in Firmicutes abundance in the CRS group was enhanced by B. adolescentis. (E) The increased Bacteroidetes abundance in the CRS group was decreased by B. adolescentis. The data are shown as the mean ± SEM. One-way ANOVA followed by the Student-Newman-Keuls test was used. *p < 0.05 vs. the control; ^#p < 0.05 vs. the CRS group; n = 3 per group. Con, Control; CRS, chronic restraint stress; Bif+CRS, B. adolescentis + chronic restraint stress.