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. 2019 Jun 18;12:155. doi: 10.3389/fnmol.2019.00155

FIGURE 5.

FIGURE 5

Normal social interaction, moderately enhanced social communication and self-grooming, and suppressed digging in heterozygous Shank3+/Q321R mice. (A) Normal social approach in Shank3+/Q321R mice (2–3 months; male) in the three-chamber test, as shown by time spent sniffing. S1, a stranger; O, object; S2, new stranger. Social novelty recognition, measured by the preference for new stranger (S2) over old stranger (S1), could not be determined due the lack of normal social novelty recognition in WT mice. n = 10 mice (WT), 13 mice (HT), ∗∗∗P < 0.001, ns, not significant, Welch’s t-test, Mann–Whitney U-test, and Student’s t-test. (B) Moderately increased courtship USVs emitted by Shank3+/Q321R mice (2–3 months; male) upon encounter with a novel female stranger, as shown by the normal number of USVs but increased mean duration of each USV calls. n = 12 mice (WT), 12 mice (HT), ∗P < 0.05, ns, not significant, Student’s t-test. (C) Enhanced self-grooming in Shank3+/Q321R mice (2–3 months; male) in home cages with bedding (10 min), as shown by total self-grooming time. n = 12 mice (WT), 13 mice (HT), ∗P < 0.05, Student’s t-test. (D) Suppressed digging in Shank3+/Q321R mice (2–3 months; male) in home cages with bedding (10 min), as shown by total digging time. n = 12 mice (WT), 13 mice (HT), ∗P < 0.05, Welch’s t-test. (E–I) Normal self-grooming in Shank3+/Q321R mice (2–3 months; male) in Laboras cages, as shown by total self-grooming duration. n = 13 mice (WT), 13 mice (HT), ns, not significant, two-way ANOVA (genotype main effect p-value = 0.4087 in E), Mann-Whitney test (F,H,I), Student’s t-test (G).