FIGURE 8.

Normal object-recognition and fear memory in homozygous male Shank3^Q321R/Q321R mice. (A) Normal levels of object recognition memory in Shank3^Q321R/Q321R mice (2–4 months; male) in the novel object recognition test, as shown by the discrimination index over a familiar object and a novel object (see Materials and Methods for details) presented 24 h after exploring two identical objects on the first day. n = 11 mice (WT), 13 mice (Q321R), ns, not significant, Student’s t-test. (B) Normal acquisition, retrieval, and extinction of contextual fear memory Shank3^Q321R/Q321R mice (3–6 months; male), as shown by freezing levels. Mice were given 5 foot shocks (2-min intervals) during the 12-min fear memory acquisition phase (day 1), and were consecutively exposed to the same context 24 h after the training (day 2) and also during days 3–9 for fear extinction. n = 13 mice (WT), 17 mice (Q321R), repeated measures two-way ANOVA. (C) Normal somatosensory function in Shank3^Q321R/Q321R mice (6 months; male) in the hot plate test, as shown by latency to first licking/jumping. n = 17 mice (WT) and 20 mice (Q321R), ns, not significant, Student’s t-test (for latency to first licking), and Mann–Whitney U-test (for latency to first jumping). (D) Normal somatosensory function in Shank3^Q321R/Q321R mice (2 months; male) in the von Frey test, as shown by threshold for response to stimulation. n = 10 mice (WT) and 11 mice (Q321R), ns, not significant, Mann–Whitney U-test.