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. 2019 Jun 25;39(6):BSR20181367. doi: 10.1042/BSR20181367

Figure 4. RMRP knockdown resulted in activation of OGD/R-induced autophagy and PI3K/Akt/mTOR-mediated apoptosis in SH-SY5Y cells.

Figure 4

(A) Immunofluorescence detection of the expression and distribution of LC3I/II. (B) Immunofluorescence detection of the expression and distribution of P62. (C) SH-SY5Y cells were cultured in the presence of vehicle, OGD/R treatment alone, OGD/R combined with NC, OGD/R combined with RMRP siRNA for 24 h, Western blot detection of the expression of LC3I, LC3II, and P62. (D) Western blot detection of activated of PI3K, Akt, and mTOR. (E) Western blot detection of Bcl-2 and Bax. β-actin was used as an internal control. All experiments were repeated at least three times. The data are presented as the mean ± S.E.M. **P<0.01.