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| Genes | Primers (5′→3′) | Tm | Fragment length |
|---|---|---|---|
| Ngn1 | |||
| Sense | CGGCCAGCGATACAGAGTCC | 59°C | 191 bp |
| Antisense | GTACGGGATGAAGCAGGGTG | ||
| Mash1 | |||
| Sense | GGCTCAACTTCAGTGGCTTC | 55°C | 291 bp |
| Antisense | TGGAGTAGTTGGGGGAGATG | ||
| NeuroD1 | |||
| Sense | TCAGTTCTCAGGACGAGGA | 59°C | 366 bp |
| Antisense | AGTTCTTGGCCAAGCGCAG | ||
| β-actin | |||
| Sense | TCACCCACACTGTGCCCATCTATGA | 56°C | 246 bp |
| Antisense | CATCGGAACCGCTCATTGCCGATAG | ||
