REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-SMC polyclonal rabbit antibody	(Lindow et al., 2002)	N/A
Anti-ParB polyclonal rabbit antibody	(Lin et al., 1997)	N/A
Anti-ScpA polyclonal rabbit antibody, affinity purified	(Wang et al., 2017)	N/A
Anti-ScpA polyclonal rabbit antibody, affinity purified	(Wang et al., 2017)	N/A
Anti-SigA polyclonal rabbit antibody	(Fujita, 2000)	N/A
Chemicals, Peptides, and Recombinant Proteins
Benzonase	EMD	Cat # 7046
Ni-NTA agarose	Qiagen	Cat # 30230
HiTrap Q HP	GE Healthcare	Cat # 17115301
Bradford Assay Kit	ThermoFisher	Cat # 23236
ATP	Dot Scientific	Cat # DSA30030–5
NADH	Acros Organic	Cat # 271100010
Phosphoenolypyruvate	Tokyo Chemical Industry	Cat # P0256
Rabbit Pyruvate Kinase	Roche Diagnostics	Cat # 10109045001
Lactate Dehydrogenase	Sigma, Darmstadt, Germany	Cat # L2500–25KU
Formaldehyde 37%	Sigma	Cat # F8775
Ready-Lyse Lysozyme	Epicentre	Cat # R1802M
HindIII	NEB	Cat # R0104M
Klenow	NEB	Cat # M0210L
Biotin-14-dATP	ThermoFisher	Cat # 19524016
T4 DNA ligase	NEB	Cat # M0202M
Proteinase K	NEB	Cat # P8107S
T4 DNA Polymerase	NEB	Cat # M0203L
Critical Commercial Assays
ATPase/GTPase Activity Assay Kit	Sigma	Cat # MAK113
NEBNext Ultra II DNA Library Prep Kit	NEB	Cat # E7645S
Deposited Data
Raw and analyzed Hi-C data	This paper	GEO: GSE95137
Original Data	Mendeley Data	http://dx.doi.org/10.17632/tygwp234gr.1
Experimental Models: Organisms/Strains
Bacillus subtilis strains, see Table S1
Oligonucleotides
See Table S3
Recombinant DNA
See Table S2
Software and Algorithms
MATLAB 8.5 (R2015)	(Wang et al., 2017)	https://www.mathworks.com/
hiclib	(Imakaev et al., 2012)	https://bitbucket.org/mirnylab/hiclib