Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Genetic manipulation in K. pneumoniae using the pCasKP-pSGKP genome editing system and the pBECKP base editing system. (A and B) Deletion of the mgrB gene in the K. pneumoniae Y4 strain (A) and of the ramR gene in the K. pneumoniae Y17 strain (B) using the pCasKP-pSGKP system. Lane CK is the PCR band from the wild-type (wt) strain as a control. (C) W301 of the tetA gene in the K. pneumoniae Y17 strain was successfully mutated to a stop codon using the pBECKP base editing system.
