FIG 3.

Borrelia burgdorferi primer pair detections and deconvolved spectral data of PCR amplicons derived from patient B. PCR/ESI-MS was performed on serial whole blood from a patient with clinically diagnosed early Lyme disease with EM. Specific primer pairs detecting B. burgdorferi isolates are plotted over time (A), displayed as cumulative detections from the four 5-ml aliquots of 20 ml blood. Primer pairs BCT6101 (leuS) (B) and BCT3511 (gyrB) (D) simultaneously detected two genotypes of Borrelia burgdorferi on day 0. (C and E) The last time points, days 2 and 8, where one genotype of B. burgdorferi was detected for these targets. Paired peaks correspond to the forward and reverse strands of the PCR amplicons, which separate under the conditions of electrospray ionization.