TABLE 1.
Details of 174 carbapenemase- and non-carbapenemase-producing bacterial organisms tested in simulated positive blood cultures by the NG-Test Carba 5 assay
| Species | Carbapenemase status (no. positive/total)a and relative meropenem susceptibility |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|
| KPC (n = 48) |
OXA-48-like (n = 21) |
VIM (n = 19)b
|
NDM (n = 15)b
|
Combination (n = 8) |
Other carbapenemase/noncarbapenemase (n = 63) |
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| Type | MIC (μg/ml) | Type | MIC (μg/ml) | Type | MIC (μg/ml) | Type | MIC (μg/ml) | Type | MIC (μg/ml) | Type | MIC (μg/ml) | |
| Klebsiella pneumoniae (total no. positive) | 40 | 15 | 11 | 7 | 6 | 27 | ||||||
| KPC-2 (15/15) | 4 to 16 | OXA-48 (15/15) | 2 to 64 | VIM-1 (8/8) | 4 to 32 | NDM-1 (7/7) | 16 to 32 | KPC-2 + VIM-1 (1/1) | 64 | CTX-M-15 (0/9) | ≤0.125 | |
| KPC-3 (15/15) | 8 to >64 | VIM-1 + SHV-12 (1/1) | 8 | KPC-3 + VIM-1 (5/5) | 32 to 64 | SHV-2a (0/5) | ≤0.125 | |||||
| KPC-3 + CTX-M-15 (10/10) | 8 to >64 | VIM-4 + CMY-4 (2/2) | 32 | SHV-5 (0/3) | ≤0.125 | |||||||
| SHV-12 (0/8) | ≤0.125 | |||||||||||
| CMY-2 (0/2) | ≤0.125 | |||||||||||
| Escherichia coli (total no. positive) | 8 | 5 | 7 | 33 | ||||||||
| KPC-3 (8/8) | 2 to 64 | OXA-48 (5/5) | 1 to 4 | NDM-1 (5/5) | 8 to 16 | CTX-M-3 (0/2) | ≤0.125 | |||||
| NDM-1 + CTX-M-15 (1/1) | 32 | CTX-M-10 (0/2) | ≤0.125 | |||||||||
| NDM-5 (1/1) | >64 | CTX-M-14 (0/5) | ≤0.125 | |||||||||
| CTX-M-2 + SHV-12 (0/2) | ≤0.125 | |||||||||||
| CTX-M-15 + impermeability (0/5) | 2 | |||||||||||
| SHV-2a (0/3) | ≤0.125 | |||||||||||
| SHV-11 (0/2) | ≤0.125 | |||||||||||
| SHV-12 (0/5) | ≤0.125 | |||||||||||
| CMY-2 (0/6) | ≤0.125 | |||||||||||
| FOX-7 + impermeability (0/1) | 4 | |||||||||||
| Enterobacter cloacae complex (total no. positive) | 1 | 5 | 2 | 1 | ||||||||
| OXA-48 (1/1) | 8 | VIM-1 (4/4) | 8 to 32 | VIM-1 + NDM-1 (2/2) | 16 to 32 | IMI-2 (0/1) | 64 | |||||
| VIM-4 + CMY-4 (1/1) | 32 | |||||||||||
| Citrobacter freundii (total no. positive) | 1 | |||||||||||
| VIM-1 (1/1) | 1 | |||||||||||
| Klebsiella oxytoca (total no. positive) | 2 | 8 | ||||||||||
| VIM-1 (1/2) | 8 | |||||||||||
| Proteus mirabilis (total no. positive) | 1 | |||||||||||
| NDM-1 (0/1) | 2 | |||||||||||
| Pseudomonas aeruginosa (total no. positive) | 2 | |||||||||||
| GES-2 (0/1) | 16 | |||||||||||
| IMP-13 (0/1) | 4 | |||||||||||
The NG-Test Carba 5 immunochromatographic assay is capable of detecting the following variants from the 5 carbapenemases (KPC, OXA-48-like, VIM, IMP, and NDM) included as targets: KPC-2/-3, NDM-1/-4/-5/-6/-7/-9, IMP-1/-8/-11, VIM-1/-2/-4/-19, and OXA-48/-181/-204/-232/-244/-517/-519/-535. Because no organism produced 1 of the detectable IMPs, the assay was unable to detect the only IMP (i.e., IMP-13) among the carbapenemases produced by the organisms in the study.
The NG-Test Carba 5 immunochromatographic assay failed to detect 2 (1VIM-1 and 1NDM-1) of the carbapenemases produced by the organisms in the study.