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. 2019 Jun;69(3):184–203. doi: 10.30802/AALAS-CM-18-000061

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Figure 7. — Vibratome sectioning for live tissue culture slices of electroporated embryonic hearts. (A) Electroporated whole hearts are embedded in a plate containing low-melting point agarose (LMPA) suspended in Ham F-12 nutrient medium. Once solidified, (B) the agarose is cut to create agarose blocks containing the electroporated hearts, which are then (C) mounted on a vibratome chuck and (D) immersed in ice-cold Locke solution to solidify the mounting glue. (E) While in ice-cold Locke solution, the agarose blocks containing the hearts are sectioned with a vibratome (F) into 300-μm-thick slices, which then (G) are transferred to a culture dish and covered with culture medium.