Table 1.
Composition of the experimental diets and supplementation levels of the enzyme products (g/kg unless otherwise stated).
| Basal diet | Protease A | Protease B | Protease C | Phytase | ||||
|---|---|---|---|---|---|---|---|---|
| Supplementation level1 | No enzyme | 25 mg/kg | 200 mg/kg | 500 mg/kg | 4000 mg/kg | 200 mg/kg | 1600 mg/kg | 1500 FTU/kg |
| Corn | 560 | 560 | 560 | 560 | 560 | 560 | 560 | 560 |
| Soybean meal2 | 371 | 371 | 371 | 371 | 371 | 371 | 371 | 371 |
| Soybean oil | 30 | 30 | 30 | 30 | 30 | 30 | 30 | 30 |
| Calcium carbonate | 19 | 19 | 19 | 19 | 19 | 19 | 19 | 19 |
| Sodium chloride | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
| Monocalcium phosphate | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
| Choline chloride | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
| Vitamin- and mineral mix3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
| TiO2 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
1The respective amount of enzyme was added on top of the mixtures; the lower supplementation of each protease product complies with the recommendations of the suppliers, the other dosage is 8-fold higher.
2Solvent-extracted soybean meal; trypsin inhibitor activity: 1.36 g/kg; urease activity: < 0.02 mg N/g (min, 30°C); nitrogen solubility index: 12%; Lys: 29.0 g/kg; reactive Lys: 24.4 g/kg (all on as-is basis).
3Provided per kg of mixed feed: vitamin A (retinyl acetate): 12,000 IE; vitamin D3 (cholecalciferol): 2,500 IE; vitamin E (dl-α-tocopherol): 50 mg; vitamin K3 (menadione): 1.5 mg; Vitamin B1 (thiamine): 2.0 mg; vitamin B2 (riboflavine): 7.5 mg; vitamin B6 (pyridoxine): 3.5 mg; vitamin B12 (cyanocobalamin): 20 μg; niacine: 35 mg; pantothenic acid: 12 mg; choline chloride: 460 mg; folic acid: 1.0 mg; biotine: 0.2 mg; iron: 80 mg; copper: 12 mg; manganese: 85 mg; zinc: 60 mg; iodine: 0.8 mg; selenium: 0.15 mg, anti-oxidant: 125 mg.