Figure 2.

AHL inducers of keyicin. (a) Six out of 96 AHLs screened for kyc cluster activation and subsequent keyicin production: 2 and 3 are natural AHLs, whereas 4–7 are synthetic. (b) Increase in keyicin production shown as positive log fold change in the absorbance at 470 nm on treatment with AHLs compared to untreated monoculture; absorbance at 470 nm also enables detection of aglycone-containing precursors to 1. Coculture with WMMA185 shown as positive control (red bar). AHLs 2 and 3 are the native LuxR signals in P. aeruginosa and V. fischeri, respectively.³