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. 2019 Feb 21;29(1):64–73. doi: 10.1093/icvts/ivz024

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© The Author(s) 2019. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.

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Figure 8: — Association between the leaflet’s extracellular matrix degeneration, elastin degradation and calcification. Trichrome (A1–D1), Elastic tissue fibres - Verhoeff-van Gieson (A2–D2) and Von Kossa staining (A3–D3) of the representative samples from each of the 4 studied groups: an uncrimped control valve (A1–A3); a calcified uncrimped valve (B1–B3); a calcified stent-crimped valve at 18-Fr; and (C1–C3) a calcified stent-crimped valve at 14-Fr (D1–D3). EVG: Elastic tissue fibres - Verhoeff's van Gieson.