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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Clin Cancer Res. 2018 Apr 24;24(15):3632–3643. doi: 10.1158/1078-0432.CCR-18-0041

Figure 2. The IDO1 pathway is activated in glioblastoma cell lines.

Figure 2.

(A) Human glioblastoma cell lines (T98G, U87, U251, MES326 and PN19) were cultured in the presence or absence of 50 ng/ml of human IFN-γ for three days and evaluated by western blot. (B) Human cell lines were cultured in 250 μM tryptophan and presence or absence of 50 ng/ml of IFN-γ. Media supernatant was collected after three days for kynurenine estimation using Ehrlich’s method. Data represent mean ± SD. (C) Orthotopic TRP tumors grown in C57BL/6 mice were analyzed for kynurenine using LC/MS and compared to normal murine brain. (D) IDO1 expression was analyzed in TRP tumors isolated from C57BL/6 mice using western blot. Results are representative of at least 3 independent experiments. *= p<0.05