Table 2.

Dissociation constants K_D, on‐ and off‐rate constants k_on and k_off for acceptor substrates, mock‐acceptor substrate, and the product B‐trisaccharide (for the color code see Scheme 1) binding to GTB from fitting a two‐state binding model to methyl TROSY titration data using the TITAN algorithm. Values in brackets are to be treated with care as the final ligand concentration was in the range of K_D. For H‐type IV trisaccharide CSPs were too small to allow for analysis.

	K D (μM)	k on (M−1s−1)	k off (s−1)
H‐Dis	164±4	1.9×107	3100±300
3DD	420±8	7.9×106	3300±200
B‐Tris	264±4	1.1×107	2900±100
H‐Tris type I	(3200±100)	(5.0×105)	(1600±200)
H‐Tris type II	291±6	7.2×105	210±7
H‐Tris type III	(2900±200)	(2.3×105)	(700±100)
H‐Tris type IV	–	–	–
H‐Tris type V	1270±20	2.0×106	2600±200
H‐Tris type VI	318±5	1.1×106	340±10