Figure 2.

Overnight phagocytosis assay of M. hyopneumoniae. Porcine alveolar macrophages were incubated with M. hyopneumoniae or E. coli overnight (16 h) and then 400 µg/mL gentamicin was applied for 6 h. Samples were plated on to solid medium to enumerate bacteria. A M. hyopneumoniae recovered from culture supernatant or disrupted macrophages, with and without gentamicin treatment. B E. coli recovered from culture supernatant or disrupted macrophages, with and without gentamicin treatment. Error bars show standard error of the mean from one experiment performed in triplicate.