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. 2019 Jun 24;50:51. doi: 10.1186/s13567-019-0667-6

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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pMHGFP-P97 plasmid diagram. Oric = M. hyopneumoniae strain 232 predicited origin of replication, EF promoter = tetM gene promoter from Enterococcus faecalis, tetM = tetracycline resistance gene, gfpmut2 = green fluorescent protein gene, P97 promoter = predicted P97 gene promoter from M. hyopneumoniae strain 232, bla = ampicillin resistance gene.