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. 2019 Jun 24;50:51. doi: 10.1186/s13567-019-0667-6

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Geometric mean fluorescence intensities of macrophages, without and without CCD, after incubation with GFP-labelled M. hyopneumoniae and E. coli. The green fluorescence intensity of PAMs following incubation with GFP-labelled M. hyopneumoniae (M. hyopneumoniae (pMHGFP-P97)) or GFP-labelled E. coli (E. coli (pKENmut2)) was measured and normalised against uninoculated PAMs. Error bars show standard error of the mean from two separate experiments, 6 replicates in total. Significant mean differences were determined by independent T-test (PAMs ± CCD and E. coli) and Mann–Whitney U test (PAMs ± CCD and M. hyopneumoniae), ***p < 0.001. Porcine alveolar macrophages (PAMs), cytochalasin D (CCD).