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. 2019 Jun 24;19:274. doi: 10.1186/s12870-019-1871-2

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Calculated average internal stability profiles of 5′ end of guide and passenger strands of miRNAs (a) at different nucleotide positions for (i) miR319g (ii) anti-miR319g (iii) miRStv_11 (iv) anti-miRStv_11; b of hexameric sequences for (i) miR319g (ii) anti-miR319g (iii) miRStv_11 (iv) anti-miRStv_11; c confirmation of selection of miR319g (Lane 1), miRStv_11 (Lane 2), anti-miR319g (Lane 3) and anti-miRStv_11 (Lane 4) strands in leaves of S. rebaudiana, transformed with knockdown constructs by the presence of ~ 50 bp bands by stem-loop RT-PCR; Lane M 10 bp ladder