Fig. 4.

Overexpression of DKK1 inhibited β-catenin protein expression in MCF-7 (a) and MDA-MB-231 (b) cells. β-actin was used as an internal reference of total protein, results are presented as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 compared to the empty vector group, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 compared to the naive group, n = 5 per group, one-way ANOVA. Naïve: untreated group, Empty: empty vector group, DKK1: DKK1 overexpressed group