Fig. 6.

Overexpression of DKK1 inhibited the mRNA and protein expression of MMP7 in MCF-7 and MDA-MB-231 cells. Real time PCR analysis suggested overexpression of DKK1 inhibited the mRNA expression of MMP7 in MCF-7 (a) and MDA-MB-231 (b) cells. Results are presented as mean ± SEM, **p < 0.01, ***p < 0.001 compared to the empty vector group, ^##p < 0.01, ^###p < 0.001 compared to the naive group, n = 5 per group, one-way ANOVA. Naïve: untreated group, Empty: empty vector group, DKK1: DKK1 overexpressed group. Western blot assay showed overexpression of DKK1 inhibited the protein expression of MMP7 in MCF-7 (c) and MDA-MB-231 (d) cells. Results are presented as mean ± SEM, **p < 0.01, ***p < 0.001 compared to the empty vector group, ^##p < 0.01, ^###p < 0.001 compared to the naive group, n = 5 per group, one-way ANOVA. Naïve: untreated group, Empty: empty vector group, DKK1: DKK1 overexpressed group