Table 4.
Most highly enriched pathways and processes for miR-146a-5p sorted by p-values
| Terms | Count | Percentage (%) | p value |
|---|---|---|---|
| KEGG pathways | |||
| Regulation of water balance by renal aquaporins | 4 | 8.696 | < 0.001 |
| Phospholipase C-mediated cascade | 4 | 8.696 | < 0.001 |
| DAG and IP3 signaling | 4 | 8.696 | < 0.001 |
| Aquaporin-mediated transport | 4 | 8.696 | < 0.001 |
| EGFR interacts with phospholipase C-gamma | 4 | 8.696 | < 0.001 |
| PLC-gamma1 signalling | 4 | 8.696 | < 0.001 |
| LPA4-mediated signaling events | 3 | 6.522 | < 0.001 |
| PKA activation in glucagon signalling | 3 | 6.522 | < 0.001 |
| PKA activation | 3 | 6.522 | < 0.001 |
| PKA-mediated phosphorylation of CREB | 3 | 6.522 | < 0.001 |
| Biological processes (BP) of GO | |||
| Transport | 15 | 12.500 | 0.014 |
| Signal transduction | 35 | 29.167 | 0.033 |
| Cell communication | 33 | 27.500 | 0.040 |
| Cellular components (CC) of GO | |||
| Transcriptional repressor complex | 2 | 2.105 | 0.005 |
| Plasma membrane | 34 | 35.789 | 0.006 |
| Actomyosin, actin part | 1 | 1.053 | 0.007 |
| Type III intermediate filament | 1 | 1.053 | 0.007 |
| Node of Ranvier | 1 | 1.053 | 0.007 |
| Symbiont-containing vacuole membrane | 1 | 1.053 | 0.007 |
| Nucleus (periNuclear) | 1 | 1.053 | 0.007 |
| Clathrin-coated vesicle | 2 | 2.105 | 0.007 |
| Activin responsive factor complex | 1 | 1.053 | 0.019 |
| Cytoplasmic vesicle | 4 | 4.211 | 0.021 |
| Molecular function (MF) of GO | |||
| Adenylate cyclase activity | 2 | 1.681 | 0.002 |
| Transcription factor activity | 12 | 10.084 | 0.009 |
| Histone binding | 1 | 0.840 | 0.020 |
| Protein-tyrosine kinase activity | 2 | 1.681 | 0.026 |
The predicted genes that overlapped in at least 7 online platforms were validated by RNA-seq data, and only 120 targets were confirmed to be inversely correlated with miR-146a-5p in expression. Enrichment analysis was conducted, adopting the 120 potential targets to identify statistically significant pathways and GO processes (p < 0.05). Most of the above terms have been reported to play essential roles in the regulation of tumorigenesis and other biological activities in cancers