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. Author manuscript; available in PMC: 2020 Jul 15.
Published in final edited form as: Int J Cancer. 2019 Jan 24;145(2):474–483. doi: 10.1002/ijc.32108

Figure 4.

Figure 4.

Inhibition of PARP activity on the effects of cytoplasmic PARP-1 on TRA-8-induced apoptosis. BxPc-3 (a, b) and MiaPaCa-2 (c, d) cells stably infected with Vector, wild-type PARP-1 (WT) or PARP-1 cytoplasmic mutants (R208Q, K222I) were treated with vehicle (Control), TRA-8 (1 μg/mL), or TRA-8 with PARP inhibitor (PJ-34, 20 μM) for 24 h. (a and c) Apoptosis was analyzed by flow cytometry. Results shown are means ± SD of three experiments performed in duplicates (*p < 0.01, compared to the TRA-8-treated cells in the Vector group or WT group; #, no difference compared to TRA-8 + PJ-34-treated cells in the Vector group or WT group). (b and d) Western blot analysis of the expression of caspase-8 (Casp8) and caspsae-3 (Casp3) in each group. The expression of β-actin was used as a loading control. Representative blots of three independent experiments are shown.