Fig. 3. Classes of proteins with respect to their abundance on NPs observed in the temperature experiment. (A) No significant changes observed (Class I); (B, C) binding affinity is reduced or enhanced at low temperatures (<30 °C, Class II); (D, E) binding affinity is reduced or enhanced at higher temperatures (>30 °C, Class III). The green curve shows the sigmoid fit. The red dotted line corresponds to the critical temperature. Whiskers correspond to standard deviation.