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. 2019 May 31;9(14):4084–4100. doi: 10.7150/thno.33638

Figure 4.

Figure 4

MSC-exos attenuated the activation of caspase-3 and caspase-12 under the AGEs stimulation in human NP cells. The human NP cells were treated with AGEs (200 μg/mL) in 24 h except for in the control group. MSC-exos-10, 50, 100 indicates that 10, 50 or 100 μg/mL exosomes were used in the corresponding groups. (A) Representative western blotting assay and quantitative analysis of cleaved caspase-3 and cleaved caspase-12 level. GAPDH was used as an internal control. (B) Representative images of caspase-3 and caspase-12 expression treated with different concentrations of MSC-exos under the stimulation of AGEs. The nuclei of NP cells were stained by DAPI. Magnification: 200 ×. (C) Quantitative analysis of fluorescence intensity for caspase-3 and caspase-12 according to the immunofluorescence analysis results. (D) Representative images of TUNEL analysis in different group. The nuclei of NP cells were stained by DAPI. Magnification: 200 ×. (E) Quantitation of the ratio of apoptotic cells in total cells was measured according to the TUNEL staining. Data were presented as the mean ± SD. *P < 0.05 vs. control group, #P < 0.05 vs. AGEs group.