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. 2019 May 31;9(14):4084–4100. doi: 10.7150/thno.33638

Figure 6.

Figure 6

MSC-exos attenuated the AGEs-induced ER stress-induced apoptosis in human NP cells. The MSC-exos group was cotreated with AGEs (200 μg/mL) and MSC-exos (100 μg/mL). (A) There fragments of si-CHOP (173, 247 and 520) were synthesized and the silencing efficiency was assessed by the western blotting analysis. Data were presented as the mean ± SD. *P< 0.05 vs. control group. (B-E) Western blot analysis and the quantitative statistical analysis showed the protein levels of CHOP (C), cleaved caspase-12 (D) and cleaved caspase-3 (E) expression. GAPDH was used as an internal control. Data were presented as the mean ± SD. *P < 0.05 vs. control group, #P < 0.05 vs. AGEs group, $P < 0.05. (F) Representative images of caspase-3, caspase-12 and CHOP protein expression were detected by immunofluorescence staining. Magnification: 200 ×. (G-H) Representative images of TUNEL staining (G) and the quantitative statistical analysis (H) showed the rate of TUNEL-positive cells in different groups. Magnification: 200 ×. Data were presented as the mean ± SD. *P < 0.05 vs. control group, #P < 0.05 vs. AGEs group, $P < 0.05.