Figure 6.
In vitro evaluation of lenti-HA in human HCC cells. (A) Measurement of IFN-γ and IL-2 in supernatant of human lymphocytes primed by lentivirus-transduced DCs with ELISA (n=3, **P<0.01). (B) Cytolysis rate against different human HCC cells with effector T cells at the E: T ratio of 10:1. A LDH-releasing cytotoxic assay was performed to measure the cytolysis efficiency of effector T cells activated by lentivirus-transduced peripheral monocyte-derived DCs (n=3, **P<0.01). (C) Cytolysis rate against human pancreatic cells with effector T cells (E: T =10:1). N.s refers to not significant. Two-tailed t test was used for statistical analysis and all the experiments were repeated twice (two repeated experiments yielded similar results and thus one representative result was shown).