Figure 3.

USP5 interacts with TUFM. A. HEK293T cells transfected with vector or Myc-USP5 plasmid were lysed and immunoprecipitated with anti-Myc antibody. The immunoprecipites were separated on SDS-PAGE and visualized with silver staining. Differential bands indicated were cut for LC-MS/MS analysis. B. Twelve peptides identified by LC-MS/MS (highlighted in yellow) were fragments of TUFM. C & D. Myc-USP5 and Flag-TUFM-expressing plasmids were transfected into HEK293T for 24 hours. Reciprocal co-immunoprecipitation and immunoblotting were performed by using anti-Flag (C) and anti-Myc antibodies (D). E & F. Whole cell lysates of HCT116 cells were subjected to reciprocal co-immunoprecipitation assays were performed by using anti-TUFM (E) or anti-USP5 antibody (F). G. The correlation between USP5 and TUFM based on data retrieved from GEPIA (http://gepia.cancer-pku.cn).