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. 2019 May 31;9(14):4208–4220. doi: 10.7150/thno.33803

Figure 5.

Figure 5

TUFM regulates colorectal cancer cell growth and is regulated by USP5. A. TUFM expression in normal and colorectal cancer tissues (http://gepia.cancer-pku.cn). B. Immunoblotting analysis of USP5 and TUFM in 8 pairs of primary colorectal cancer and non-cancerous tissues. GAPDH was used as a loading control. C. The viability of HCT116 cells transfected with Flag-TUFM-expressing plasmid or empty vector (EV) were assessed by CCK-8 staining at day 0, 1, 2 and 4. Immunoblotting was performed to determine the expression levels of Flag-TUFM, Cyclin D1 and GAPDH at day 4. *p<0.05, **p<0.01. D. The viability of HCT116 cells transfected with siTUFM#1, siTUFM#2, siTUFM#3 or control were measured by CCK-8 staining at day 0, 2, 4 and 6. Immunoblotting assay was performed to examine the expression of TUFM, Cyclin D1 and GAPDH at day 4. *p<0.01. E & F. HCT116 cells infected with lentiviruses expressing shUSP5#3 or transfected with Flag-TUFM-expressing vectors were assessed by CCK-8 staining (E) and Immunoblotting analyses (F). G. Quantitative and statistical analysis of Cyclin D1 expression from F. H. HCT116 cells treated with indicated concentrations of WP1130 for 12 hours were examined by immunoblotting against USP5, TUFM and GAPDH.