Figure 2.

The inhibitory effects of overexpressing RACK1 on cell viability in HGC27 and MGC803 cells. qRT-PCR (A) and Western blot (B) were used to detect the transfection efficiency of overexpressing RACK1 in HGC27 cells. (C) The effect of overexpressing RACK1 on cell viability at 12, 24, and 48 h was detected by CCK-8 assay in HGC27 cells. qRT-PCR (D) and Western blot (E) were used to detect the transfection efficiency of overexpressing RACK1 in MGC803 cells. (F) The effect of overexpressing RACK1 on cell viability at 12, 24, and 48 h was detected by CCK-8 assay in MGC803 cells. Expression of each protein in cells was following normalization with a loading control GAPDH. Data are shown as mean ± SD in three independent experiments.

Notes: Compared with control, *P<0.05, **P<0.01; compared with NC ^{^}P<0.05, ^{^^}P<0.01.

Abbreviations: qRT-PCR, quantitative real-time polymerase chain reaction; CCK-8, cell counting kit-8; NC, negative control.