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HER2 mutations cause oncogenic transformation of MCF10A. (a) Structure visualizations of the HER2 somatic mutations, TM, transmembrane region. (b) Western blotting showing the expression of HER2 in indicated MCF10A cells. β-actin was used as a loading control. (c) Proliferation data for indicated MCF10A cells grown in monolayer for 4 days. (D) Soft agar assay of indicated MCF10A cells for 14 days. Data are expressed as means ± SEM of three technical replicates.
