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. 2019 May 14;18(10):1110–1121. doi: 10.1080/15384101.2019.1609829

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Figure 4. — The oncogenic activity of CCAT1 partially negatively regulates miR-181a-5p and then modulates MMP14 and HBEGF. (a) Cell viabilities of the SiHa and HeLa cells transduced with lv-CCAT1 and then transfected with miR-181a-5p mimic or (b) lv-shCCAT1 and then transfected with miR-181a-5p inhibitor. The cell viability was measured by the CCK-8 assay. (c) The relative mRNA expression of CCAT1, MMP14 and HB-EGF in SiHa and HeLa cells transduced with lv-CCAT1 and then transfected with miR-181a-5p mimic or (d) transduced with lv-shCCAT1 and then transfected with miR-181a-5p inhibitor. (e) The protein expression of MMP14 and HB-EGF in SiHa and HeLa cells transduced with lv-CCAT1 and then transfected with miR-181a-5p mimic or (f) transduced with lv-shCCAT1 and then transfected with miR-181a-5p inhibitor. Lv-CCAT1 and lv-con, CCAT1 overexpression lentivirus and its control lentivirus, respectively. Lv-shCCAT1 and lv-shcon, CCAT1 knockdown lentivirus and its control lentivirus, respectively.