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. 2019 Jun 16;10(1):261–270. doi: 10.1080/21655979.2019.1631104

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Inhibition of XIST by shRNA blocks proliferation and induces apoptosis in U2OS cells. A, U2OS cells were transfected with Lv-shRNA and Lv-scramble for 24 h-96 h. Relative expression of XIST in U2OS cells was detected by qRT-PCR. Data were shown using 2^−ΔCT values. GAPDH was used for normalization. B, The MTT assay revealed dose-dependent inhibition of cell proliferation after XIST shRNA treatment was applied to U2OS cells. C, The results of Annexin V assay combined with propidium iodide assays showed that inhibition of the Xist promoted U2OS cells apoptosis. Data are represented as mean±SD of three independent experiments.D, The results of TUNEL assays showed that inhibition of the Xist promoted U2OS cells apoptosis. Data are represented as mean±SD of three independent experiments.^aP<0.05;^bP<0.01.