Table 3.
Primers | Sequence (5′-3′) | Source of references |
---|---|---|
Tup | CCGCGTCGACAATTCTTTATCCAATGGGAT (SalI) | This work |
Tdown | GGTGTGAGGACGTTAAAGAG (1032bp) | |
Sup | CTCTTTAACGTCCTCACACCTTTCAAGAACATGTCCAAAT | This work |
Sdown | ACCGGAATTCAAAAGGTCTGAGTTGACGAA (EcoRI)(958bp) | |
pdh-X | CCAATGGGATAAGGGTA | This work |
pdh-Y | TGTTTCATTGACGAGTAAA (2685 bp) | |
pdh-ORF-S | CATGATGGCTGAGCTTGC | This work |
pdh-ORF-A | TAAGCGTGTTCTTTCGGG (681 bp) | |
pdh-F | CGCGAATTCATGCAACAAATCCGTGAT (EcoRI) | This work |
pdh-R | CCCTCGAGGCTAGTCTACAAACACATC (XhoI) (924bp) |