Fig. 3.

Relative rates of NIC-OH and NIC-G formation in liver and intestinal microsomes of wild-type, LCN, and IECN mice. The relative rates of NIC-OH (A) and NIC-G (B, C) formation in hepatic (A and B) and intestinal (C) microsomes were derived from chromatographic peak area ratios of the metabolites to that of an internal standard. Formation of NIC-OH was detected with hepatic microsomes (A), but not intestinal microsomes (data not shown). The reaction mixtures contained 0.1 mg microsomal protein, 100 µM niclosamide, 0.1 M potassium phosphate buffer (pH 7.4), 1.0 mM NADPH or 5.0 mM UDPGA, and 3 mM MgCl₂ in a final volume of 200 µl. The incubation was carried out at 37°C for 30 minutes. The values represented means ± S.D., n = 3–5; *P < 0.05; **P < 0.01; ****P < 0.0001, compared with wild-type group; one-way analysis of variance with Bonferroni’s multiple comparisons test.