Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Feb 1;95(2):430–438. doi: 10.3382/ps/pev365

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Poultry Science Association Inc.

PMC Copyright notice

Figure 1. — The neutralizing effects of affinity purified egg yolk anti-IL-10 antibody were determined by measuring the ability of recombinant chicken IL-10 to inhibit IFN-γ production in Concanavalin A (Con A) stimulated chicken splenocytes. Splenocytes were untreated, stimulated with 20 μg Con A, stimulated with Con A and 0.2 ng/mL, recombinant chicken IL-10, or stimulated with Con A, IL-10, and 0.85 μg/mL affinity purified egg anti-IL-10 specific antibody. Culture media was analyzed for IFN-γ 24 hours after treatment. Anti-IL-10 was effective at neutralizing IL-10s inhibition of IFN-γ release (overall ANOVA P < 0.0001, standard error of the mean bars n = 3). ^a–dTreatment means with different letter superscripts are statistically different (P < 0.05).