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. 2019 Jun 24;2(3):e201900402. doi: 10.26508/lsa.201900402

Figure S5. Efficiency of Cre recombinase–mediated 3′UTR excision in TgATPaseP-GC mutant.

Figure S5.

Efficiency of 3′UTR excision was quantified by scoring the loss of HA signal in the TgATPaseP-GC-HA3’IT-3′UTRexcised mutant, which was generated as described in Fig 4, and immunostained with α-HA and α-TgGap45. 300 vacuoles containing parasites with or without HA signal were counted from three independent IFAs (mean with SEM).