Table 1. Rate of thioester hydrolysis of TEP1cut.
TEP1 form | t1/2 (h) | Ref. |
---|---|---|
*S2 | 4.2 ± 0.2 | this work |
*S1 | 8.5 ± 0.2 | [8] |
*R2 | 9.6 ± 0.4 | this work |
*R1 | 156 ± 12 | [8] |
*R1 S1cat | 9 ± 2 | this work |
*R1 N919G | 55 ± 10 | this work |
Half-life for thioester hydrolysis determined by rate of precipitation for cleaved forms of TEP1. Each half-life is determined from three independent experiments.