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. 2019 Jun 25;14(6):e0218968. doi: 10.1371/journal.pone.0218968

Fig 3. Expression of CML-susceptible gene RMND1 is affected by TF RFX3 binding affinity.

Fig 3

(A) The CML-associated SNP rs6931104 affects the binding affinity of transcription factor RFX3. ChIP-ed DNA enrichment determined by qRT-PCR is graphed as the amount of immunoprecipitated DNA. Error bars indicate SD values. (B) Effects of RFX3 knockdown (KD) or overexpression (OV) on RMND1 transcript levels in BCR-ABL1+ cells. Total RNA expression was assayed by RT-qPCR. Asterisk marked p-values were calculated by comparing with control in each of the three cell lines, respectively. **p < 0.01, *p < 0.05, ns–not significant. Error bars indicate SD values. (C) RMND1 transcript levels in CML patients. Total RNA expression was assayed by RT-qPCR. Minus delta Ct (GAPDH Ct value–RMND1 Ct value) indicates the expression levels (Y-axis) in samples from 12 human CML patients and 12 healthy individuals. All statistical significance was evaluated by the Student’s t-test.