Table 2.
Experimental groups for MPTP studies
| Group | Treatment Description | Figures |
|---|---|---|
| MPTP + CLFB-1134 (Co-dosed) |
Mice received 20mg/kg MPTP once per day (s.c.; 1:00pm) and 30mg/kg CLFB-1134 twice per day (i.p.; 8:00 am and 8:00 pm) for 5 consecutive days. Sacrifice was 3 hours after the final MPTP dose. | Figure 2 |
| MPTP + drug vehicle (Co-dosed) |
Mice received 20mg/kg MPTP once per day (s.c.; 1:00pm) and DMSO/PEG vehicle twice per day (i.p.; 8:00 am and 8:00 pm) for five consecutive days. Sacrifice was 3 hours after the final MPTP dose. | |
| Neurotoxin vehicle + CLFB-1134 | Mice received a single i.p. injection of CLFB-1134 and were given s.c. saline 30 minutes later. | Figure 3 |
| Neurotoxin vehicle + drug vehicle | Mice received a single i.p. injection of DMSO/PEG vehicle and were given s.c. saline 30 minutes later. | |
| MPTP + drug vehicle | Mice received a single i.p. injection of DMSO/PEG vehicle and were given s.c. MPTP 30 minutes later. | |
| MPTP + CLFB-1134 | Mice received a single i.p. injection of CLFB-1134 and were given s.c. MPTP 30 minutes later. | |
| Neurotoxin vehicle | Mice received a single daily injection of saline (s.c.) for 5 consecutive days. Mice were sacrificed 3 weeks following the last injection. | Figures 4 and 5 |
| MPTP | Mice received a single daily injection of 20 mg/kg MPTP (s.c.) for 5 days in a row. Mice were sacrificed 3 weeks following the last injection. | Figures 4, 5, and 6 |
| MPTP + CLFB-1134 (Co-dosed) |
Mice were treated with a single daily injection of 20 mg/kg MPTP (s.c.) and two daily injections of CLFB-1134 (i.p.) for 5 days. CLFB-1134 injections continued on the same schedule for 3 more weeks following MPTP completion. Mice were sacrificed following the last CLFB-1134 injection. | Figures 4, 5, and 6 |
| MPTP + drug vehicle (Co-dosed) |
Mice were concurrently treated with a single daily injection of 20 mg/kg MPTP (s.c.) and two daily injections of DMSO/PEG (i.p.) for 5 days. DMSO/PEG injections continued on the same schedule for 3 more weeks. Mice were sacrificed following the last injection. | Figures 4 and 5 |
| MPTP + CLFB-1134 (Post MPTP) |
Mice received a single daily injection of 20 mg/kg MPTP (s.c.) for 5 consecutive days. Mice then received two daily injections of CLFB-1134 (i.p.) for 3 weeks. Mice were sacrificed following the last injection. | Figures 4, 5, and 6 |