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. 2019 Jun 25;8:e44199. doi: 10.7554/eLife.44199

Figure 6. Ribosome rescue allows UPR signaling from the HAC1 5’ exon fragment.

Figure 6.

(A) Evaluating the role of ribosome recycling on the ski2Δ-dependent growth rescue of trl1-H148Y cells under ER stress. Serial five-fold dilutions of trl1-H148Y cells with the indicated deletions of SKI2 and/or DOM34 were spotted on YPD plates without (-Tm) or with (+Tm) 0.1 μg/ml tunicamycin and incubated at 30°C for 2 days. (B) Lysates of the indicated strains were prepared and immunoblotted for 3xHA-Hac1p and GAPDH as loading control. Full-length Hac1p is indicated by a closed arrowhead, truncated Hac1p by an open arrowhead. An even shorter minor third band is marked with an asterisk. The high molecular weight smear/banding pattern is indicated as HMW-Hac1p.